By Nathan P. Kaplan, Nathan P. Colowick, C. H.W. Hirs, Serge N. Timasheff

The seriously acclaimed laboratory normal, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. seeing that 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this present day - actually a necessary ebook for researchers in all fields of existence sciences

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These crystals are subjected to the recrystallization described in operation 1. Refractive Index and Concentration The differences (AN) between the refractive index of a GuHC1 solution (N,) and that of water (N ° ) are listed in Table IV. We prefer the difference AN to the absolute value ND, since systematic errors due to the individual person or instrument will cancel out. The experimental data have been accmnulated for the past several years, using an Abbe refractometer (hence the refractive index for the sodium D line is obtained).

H. Miller, Proc. Natl. Acc~d. Sci. S. 69, 897 (1972). 28 MOLECULAR WEIGHT DETERMINATIONS [2] [2] Protein Polypeptide Chain Molecular Weights by Gel Chromatography in Guanidinium Chloride By KENNETH G. MANN and WAYNE W. FISH Because gel chromatography is simple and inexpensive, it has become a common method for the separation of biological maeromolecules according to size. Gel chromatographic separations are achieved by means of the differential distribution of solute molecules between the stationary solvent within the pores of a gel and the mobile eluting solvent outside the pores.

001%. Fluorescein labeling is most easily accomplished with fluorescein isothiocyanate on Celite (Calbiochem, Los Angeles). After reduction and carboxymethylation of the sample in GuHC1, sufficient solid NaHCO3 is added to bring the concentration to approximately 1% in this reagent. The labeling reaction is initiated by the addition of 10 mg of 10% fluorescein isothiocynate on Celite per milliter of solution. 0 by the addition of 1 N HC1. The solution is then degassed under vacuum, and blue dextran is added.

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