By Martin Chalfie, Steven R. Kain
Because the discovery of the gene for eco-friendly fluorescent protein (GFP), derived from jellyfish, this protein that emits a eco-friendly glow has initiated a revolution in molecular biosciences. With this software, it's now attainable to imagine approximately any protein of curiosity in any mobile or tissue of any species. because the ebook of the 1st version, there were drastically major technological advances, together with improvement of latest mutant editions. Proteins at the moment are to be had in yellow and blue, and Novel Fluorescent Proteins (NFPs) have improved their software in constructing biosensors, organic markers, and different organic functions. This up-to-date, multiplied new version locations emphasis at the upward thrust of NFPs, together with new chapters on NFP houses with distinctive protocols, functions of GFPs and NFPs in study, and biosensors. This publication presents an effective theoretical framework, besides certain, sensible information on use of GFPs and NFPs with dialogue of power pitfalls. The specialist members supply genuine examples in exhibiting the right way to tailor GFP/NFP to express structures, maximize expression, and increase detection.
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Extra info for Green Fluorescent Protein: Properties, Applications and Protocols
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2000. 1. Stable transgenic zebrafish expressing GFP in specific tissues. , 1997). (C, D) GATA-2 BAC GFP expression in neuronal cells (Shuo Lin, unpublished). , 1999). , 1999). , 2001). , 2003). , 2003). 2. GFP expression in transgenic mouse and chick. (A, B) GFP expression in neural tube and neural crest following electroporation of chick with a GFP encoding plasmid (green) and anti-HNK1 immunostaining (red) to mark neural crest. (A) Lateral view of whole mount (Maria Elena de Bellard and Marianne Bronner-Fraser, unpublished).