By Martin Chalfie, Steven R. Kain

Because the discovery of the gene for eco-friendly fluorescent protein (GFP), derived from jellyfish, this protein that emits a eco-friendly glow has initiated a revolution in molecular biosciences. With this software, it's now attainable to imagine approximately any protein of curiosity in any mobile or tissue of any species. because the ebook of the 1st version, there were drastically major technological advances, together with improvement of latest mutant editions. Proteins at the moment are to be had in yellow and blue, and Novel Fluorescent Proteins (NFPs) have improved their software in constructing biosensors, organic markers, and different organic functions. This up-to-date, multiplied new version locations emphasis at the upward thrust of NFPs, together with new chapters on NFP houses with distinctive protocols, functions of GFPs and NFPs in study, and biosensors. This publication presents an effective theoretical framework, besides certain, sensible information on use of GFPs and NFPs with dialogue of power pitfalls. The specialist members supply genuine examples in exhibiting the right way to tailor GFP/NFP to express structures, maximize expression, and increase detection.

Show description

Read Online or Download Green Fluorescent Protein: Properties, Applications and Protocols PDF

Similar clinical chemistry books

Carbon-rich compounds: from molecules to materials

The 2 easy development devices carbon and hydrogen will be mixed in 1000000 alternative ways to provide a plethora of interesting natural compounds. Henning Hopf provides not just the main notable constructions and homes of hydrocarbon compounds yet indicates in a transparent presentation and with nice didactic ability how molecules like dodecahedrane, superphane or annulenes problem the bogus talents of each natural chemist.

Bioactive Marine Natural Products

Marine usual items have attracted the eye of biologists and chemists internationally for the previous 5 many years. due to the strength for brand spanking new drug discovery, marine normal items have attracted scientists from assorted disciplines, comparable to natural chemistry, bioorganic chemistry, pharmacology, biology and ecology.

Lignin and Lignans: Advances in Chemistry

During the last 4 many years, there was sizeable development in each sector of lignin technological know-how, starting from the enzymology of lignin biodegradation, to the delignification of wooden fiber in the course of pulping and bleaching, to advances in spectroscopy. Lignin and Lignans: Advances in Chemistry captures the advancements which have been completed through world-class scientists within the most crucial features of this burgeoning box.

Handbook of Thermal Analysis and Calorimetry, Volume 2: Applications to inorganic and miscellaneous materials (Handbook of Thermal Analysis and Calorimetry)

This is often the second one quantity of a 4 quantity set meant to explain the options and functions of thermoanalytical and calorimetric equipment. the overall ideas and technique are lined generally in quantity 1, besides the basic physicochemical history wanted. for this reason the next volumes reside at the purposes of those strong and flexible equipment, whereas assuming a familiarity with the recommendations.

Extra info for Green Fluorescent Protein: Properties, Applications and Protocols

Sample text

1953). The Medusae of the British Isles, Vol. I: Anthomedusae, Leptomedusae, Limnomedusae, Trachymedusae and Narcomedusae, Cambridge University Press, London, 530 pages. Shimomura, O. (1979). Structure of the chromophore of Aequorea green fluorescent protein. FEBS Lett. 104:220–222. Shimomura, O. (1984). Bioluminescence in the sea: photoprotein systems. Symp. Soc. Exp. Biol. 39:351–372. Shimomura, O. (1995a). A short story of aequorin. Biol. Bull. 189:1–5. Shimomura, O. (1995b). Cause of spectral variation in the luminescence of semisynthetic aequorins.

Bull. Fish Aquat. Sci. Bull. 204:1–181. , Ward, W. , and Prasher, D. C. (1994). Green-fluorescent protein as a marker for gene expression. Science 263:802–805. Cody, C. , Prasher, D. , Westler, W. , Prendergast, F. , and Ward, W. W. (1993). Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry 32:1212–1218. Cormier, M. , Karkhanis, Y. , Anderson, J. , Wampler, J. , Morin, J. , and Hastings, J. W. (1973). Evidence for similar biochemical requirements for bioluminescence among the coelenterates.

2000. 1. Stable transgenic zebrafish expressing GFP in specific tissues. , 1997). (C, D) GATA-2 BAC GFP expression in neuronal cells (Shuo Lin, unpublished). , 1999). , 1999). , 2001). , 2003). , 2003). 2. GFP expression in transgenic mouse and chick. (A, B) GFP expression in neural tube and neural crest following electroporation of chick with a GFP encoding plasmid (green) and anti-HNK1 immunostaining (red) to mark neural crest. (A) Lateral view of whole mount (Maria Elena de Bellard and Marianne Bronner-Fraser, unpublished).

Download PDF sample

Rated 4.55 of 5 – based on 45 votes