By Fan Lin, Jeffrey Prichard (eds.)
In a conceptually present, quick-reference, query & resolution layout, the second one variation of Handbook of useful Immunohistochemistry: commonly asked Questions continues to supply a entire and but concise state of the art evaluation of the main concerns particular to the sector of immunohistochemistry. With hyperlinks to the authors Immunohistochemical Laboratory web site, this quantity creates a present and up to date info procedure on immunohistochemistry. This comprises entry to tissue microarrays (TMA) of over 10,000 tumors and basic tissue to validate universal diagnostic panels and supply the simplest reproducible information for diagnostic purposes.
Fully revised and up to date from the 1st variation, the hot gains of the second one variation contain over two hundred extra questions or revised questions with an IHC panel to respond to each one query; over 250 new colour photographs and illustrations; over 20 new valuable biomarkers; 1000s of recent references; numerous new chapters to hide phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, assessment of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who're foreign specialists in a comparable box; many up-to-date IHC panels utilizing Geisinger IHC facts accumulated from over 10,000 tumors and general tissues; and up-to-date appendices containing exact antibody details for either guide and automatic staining procedures.
Comprehensive but sensible and concise, the Handbook of useful Immunohistochemistry: commonly asked Questions, moment version will be of serious price for surgical pathologists, pathology citizens and fellows, cytopathologists, and cytotechnologists.
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Additional info for Handbook of Practical Immunohistochemistry: Frequently Asked Questions
2009;9:409. 21. Dumur CI, Sana S, Ladd AC, et al. Assessing the impact of tissue devitalization time on genome-wide gene expression analysis in ovarian tumor samples. Diagn Mol Pathol. 2008;17(4):200–6. 22. Hopwood D. Fixatives and ﬁxation: a review. Histochem J. 1969;1(4):323–60. 23. Mason JT, O’Leary TJ. Effects of formaldehyde ﬁxation on protein secondary structure: a calorimetric and infrared spectroscopic investigation. J Histochem Cytochem. 1991;39(2):225–9. 24. Medawar PB. The rate of penetration of ﬁxatives.
If Strong Staining Signal and Strong Background Staining Is Obtained – – – – – Reduce the primary antibody concentration. Shorten the primary antibody incubation time. Shorten the secondary antibody incubation time. Further block the background staining. Try different antigen retrieval methods. If Weak Staining Signal and No Background Staining Is Obtained – – – – – Increase the primary antibody concentration. Increase the incubation time for the primary antibody. Increase the incubation time for the secondary antibody.
Procedure 1) Fill a Coplin staining jar or other suitable container with a sufﬁcient quantity of target retrieval solution. Place the container in a water bath. Heat the water bath to 95–99 °C (do not boil). 2) Dewax the sections in two changes of xylene, 5 min each. 3) Rehydrate the slides in two changes of 100 % and 95 % ethanol for 30 s each and 70 % ethanol for 30 s; then rinse in distilled water. 4) Incubate the sections in preheated target retrieval solution in a water bath for 20 min. 5) Remove the entire jar or container with slides from the water bath and allow the slides to cool for 20 min at room temperature.