By Takayuki Shibamoto
Concentrating on cutting-edge fuel chromatography (GC) and high-performance liquid chromatography, this consultant discusses the theories at the back of, and purposes of, the most recent advancements in chromatographic innovations - assessing the strengths and obstacles of every methodology.;Presenting many detection tools for the 1st time, Lipid Chromatographic research: offers a assessment of traditional column and thin-layer chromatography; examines a brand new GC strategy to observe plasmalogen phospholipids; outlines a supercritical fluid chromatography for lipids; info the 1st systematic description of a microanalytical strategy for ldl cholesterol and similar compounds; explains a GC research of lipid breakdown items for tracking organic procedures comparable to aging, carcinogenesis and mutagenesis; and describes recommendations that permit for the simultaneous research of non-polar and polar lipids
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H... 06/05/2007 Document Página 1 de 1 Page 34 dichloromethane/acetone/acetonitrile mixtures into six fractions of increasing unsaturation: saturated FAMEs, monoenes, dienes, trienes, tetraenes, pentaenes, and hexaenes. Recoveries were not reported; however, cross-contamination was only 2 to 5% between the fractions, as determined by GC. The extent of cross-contamination was dependent on the composition of the original oil. The fractions were not contaminated with silver ions as has previously been observed with silver ion TLC applications.
The two methods gave similar results. 2%) and total recovery ranged from 82 to 98%, depending on the oil. Capacity was approximately 90 mg of oil using columns packed with 500 mg of sorbent. Breakthrough of the nonpolar fraction into the polar fraction occurred when greater than 110 mg of sample was applied. A similar procedure was used by Nash and Frankel (1986) to fractionate crude soybean oil into three fractions: neutral lipids (including triglycerides), nonsaponifiables, and phospholipids.
1990. Silver ion chromatography of triacylglycerols on solid phase extraction columns packed with a bonded sulphonic acid phase. J. Sci. Food Agric. 52: 573–577. Christie, W. , Noble, R. , and Davies, G. 1987. Phospholipids in milk and dairy products. J. Soc. Dairy Technol. 40(1): 10–12. , Léger, C. , and Viret, J. 1989. Modification of fluidity and lipid–protein relationships in pig intestinal brush-border membrane by dietary essential fatty acid deficiency. Biochim. Biophys. Acta 980: 77–84. Coleman, R.