By Elliot M. Meyerowitz (auth.), Gloria Coruzzi, Pere Puigdomènech (eds.)
Presented here's an research of plant improvement and plant metabolism utilizing the instruments of genetics and molecular biology, reminiscent of mutant research, mutation tagging, mapping utilizing polymorphic characters and easy molecular biology thoughts. experiences with a number of version genetic organisms, such a lot particularly maize and Arabidopsis, are integrated. The reader earnings a complete view of the topic that's progressively more of either medical and commercial curiosity. the price of simple study in vegetation is highlighted and examples the place easy reviews have resulted in functions in agricultural biotechnology are given.
Read or Download Plant Molecular Biology: Molecular Genetic Analysis of Plant Development and Metabolism PDF
Similar analysis books
Analysis of Reliability and Quality Control: Fracture Mechanics 1
This primary publication of a 3-volume set on Fracture Mechanics is principally based at the significant diversity of the legislation of statistical distributions encountered in a variety of clinical and technical fields. those legislation are crucial in figuring out the likelihood habit of elements and mechanical constructions which are exploited within the different volumes of this sequence, that are devoted to reliability and qc.
- Multidimensional shielding analysis of the JASPER in-vessel fuel storage experiments
- Agriculture, Growth and Redistribution of Income: Policy Analysis with a General Equilibrium Model of India
- Author Cocitation Analysis: Quantitative Methods for Mapping the Intellectual Structure of an Academic Discipline (Premier Reference Source)
- Geographic Information Analysis, Second Edition
- Inelastic Behaviour of Structures under Variable Repeated Loads: Direct Analysis Methods
Additional resources for Plant Molecular Biology: Molecular Genetic Analysis of Plant Development and Metabolism
Sample text
Here we will focus on one aspect of root morphogenesis - cell expansion. The physiology of cell expansion during cell growth has been studied extensively (Taiz, 1984; Cosgrove and Kniefel, 1987). , 1993) Our goal it to use a genetic approach to dissect the processes involved in cell expansion in roots of Arabidopsis thaliana and to reveal the mechanistic and genetic basis for specific expansion patterns. , 1993). NATO ASISeries, Vol. H 81 Plant Molecular Biology Edited by G. Corum and P. Puigdommech Ii:) Springer-Verlag Berlin Heidelberg 1994 32 MATERIAL AND METHODS Plant strains.
Hypocotyl of the seedling. Such a model predicts that the complete root derives from the meristematic initials. In order to test which of these alternative developmental scenarios is happening, we performed clonal analysis. ), were used. These plants contain a T-DNA insert containing a 35S CaMV promoter, a maize Ac element, and B-glucuronidase (GUS) coding sequences. Upon excision of the Ac element in embryonic cells, clones of descendant cells can be formed in which GUS activity can be histologically detected.
1993). , 1993). Crosses were performed as described by Benfey et al, (1993). Growth measurements and cell area calculations For measurement of the root length, seedlings were photographed at roughly 24 hour intervals with a 35 rnrn camera under a Nikon stereo microscope, using Kodak Ektachrome 160T film. The root length was measured from the projected slides with a curvimeter (run-mate TM, Holland). 7%) : ethanol (50%) : acetic acid (5%» and mounted with glycerol on microscope slides. Phenotypes were analyzed with a Leitz Laborlux S compound microscope.