By William L. Hochfeld
Containing authoritative and in-depth insurance, generating Biomolecular fabrics utilizing Fermenters, Bioreactors, and Biomolecular Synthesizers examines the bioproduction platforms that help the managed, automatic, and volume progress of proteins. The e-book discusses the substance, personality, make-up, and caliber of the fundamental fabrics utilized in the creation and downstream processing of boimolecular fabrics: uncooked fabrics, reagents, intermediates, and consumables. Dr. Hochfield will get correct to the purpose, explaining simply what needs to be performed and the way to do it successfully, then supplying the formulation worthy for achieving the necessary worth, permitting you to easily plug-in your information and make protein. notwithstanding, if you happen to really do want the starting place and derivation of any given formulation, you could cross correct to the broad reference part within the Appendix, locate the formulation you would like within the special shape that you just desire it, with no need to plow through various pages of extraneous fabric. This vintage paintings offers unparalled, specified, and state-of-the-art info on bioprocessing structures. A operating reference and formulary for generating recombinant, bioactive, or different unique proteins, peptides, and nucleic acids to specification, the textual content offers assurance of the similar applied sciences, coupled with the huge biotechnology thesaurus, manufacturer’s directories, broad references, vital formulae, charts, illustrations, finished index, emphasis on sensible strategies, time-proven tools, and crucial functions. those positive aspects mix with its creative, easy-to-use structure to make it the source you'll seek advice frequently.
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Extra info for Producing Biomolecular Substances with Fermenters, Bioreactors, and Biomolecular Synthesizers
Sample text
Acetylation) or by covalent attachment of ligands, although the most accurate and predictable modifications are achieved by site-directed mutagenesis of the gene encoding the protein, followed by its expression in a suitable host organism. Such advances in DNA technology have enabled the cloning of genes isolated directly from bacterial sources or via mRNA from higher organisms into expression vectors to produce both native and mutated proteins. CELL LINES Cell-line expression technology is crucial to the success of cell-culture production.
These cells typically reach production levels per day of 3-6 × 1014 molecules of heterologous protein per 106 cells. High expression levels have also been achieved with bovine papillomavirus (BPV) vectors in mouse C127 cells, with protein production per day ranging from 1012 to 1013 molecules of heterologous protein per 106 cells. fm Page 23 Tuesday, May 16, 2006 9:37 AM Technology Overview INTRA- VS. 23 EXTRACELLULAR EXPRESSION There are two basic modes of expression: (1) intracellular, in which the heterologous protein accumulates within the host-cell cytoplasm, either as a soluble protein or an insoluble aggregate; and (2) extracellular, in which the heterologous protein genetic sequence has been manipulated to secrete the protein into the culture medium.
Cell-line productivity varies enormously with the host cell type, and it can be determined whether the cells will only successfully grow when attached to a solid surface (anchor-dependent) or in free suspension in a liquid (anchor-independent). The vast majority of cell-products are either monoclonal antibodies from hybridoma cell lines, or recombinant products, typically expressed in E. coli. Both cell types can be readily grown in suspension culture by careful attention to media and fermenter/bioreactor design.