By Michael Kinter
The best way to layout, execute, and interpret experiments for protein sequencing utilizing mass spectrometryThe speedy enlargement of searchable protein and DNA databases lately has brought on an explosive development within the program of mass spectrometry to protein sequencing. This well timed and authoritative ebook presents pros and scientists in biotechnology examine with whole assurance of systems for interpreting protein sequences via mass spectrometry, together with step by step directions for pattern coaching, research, and knowledge interpretation.Michael Kinter and Nicholas Sherman current their very own high quality, laboratory-tested protocols for the research of a large choice of samples, demonstrating easy methods to perform particular experiments and acquire speedy, trustworthy effects with a ninety nine% good fortune fee. Readers gets enough experimental aspect to use of their personal laboratories, know about the correct choice and operation of tools, and achieve crucial perception into the basic ideas of mass spectrometry and protein sequencing. assurance includes:Peptide fragmentation and interpretation of product ion spectraBasic polyacrylamide gel electrophoresisPreparation of protein digests for sequencing experimentsMass spectrometric research utilizing capillary liquid chromatographyTechniques for protein identity through database searchesCharacterization of converted peptides utilizing tandem mass spectrometryAnd even more
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Additional info for Protein Sequencing and Identification Using Tandem Mass Spectrometry
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