By Tan, Seang Lin
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Extra info for In Vitro Maturation of Human Oocytes
Sample text
Developmental pattern of the secretion of cumulus expansion-enabling factor by mouse oocytes and the role of oocytes in promoting granulosa cell differentiation. Devel Biol 1990; 140: 307–17. 33. Vanderhyden BC, Telfer EE, Eppig JJ. Mouse oocytes promote proliferation of granulosa cells from preantral and antral follicles in-vitro. Biol Reprod 1992; 46: 1196–204. 34. Motro B, Bernstein A. Dynamic changes in ovarian c-kit and Steel expression during the estrous reproductive cycle. Devel Dyn 1993; 197: 69–79.
An increased lactate output would increase the lactate concentration in follicular fluid, so pushing down the pH. It has previously been shown that meiotic maturation of the mouse oocyte– cumulus complex is suppressed in more acidic conditions69. In conditions affecting aspects of metabolism, such as diabetes or polycystic ovarian syndrome, follicle metabolism may be perturbed. Hexokinase normally phosphorylates glucose forming glucose-1-phosphate, which can then be metabolized through glycolysis or through the pentose phosphate pathway.
Miro F, Hillier SG. Modulation of granulosa cell deoxyribonucleic acid synthesis and differentiation by activin. Endocrinology 1996; 137: 464–8. 66. Joyce IM, Pendola FL, Wigglesworth K et al. Oocyte regulation of Kit Ligand expression in mouse ovarian follicles. Devel Biol 1999; 214: 342–53. 67. Laitinen M, Rutanen EM, Ritvos O. Expression of c-kit ligand messenger ribonucleic acids in human ovaries and regulation of their steady state levels by gonadotropins in cultured granulosaluteal cells.