By Kenneth M. Smith C.B.E., D.Sc., Ph.D., F.R.S. (auth.)

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The resultant mixture is poured into large separating funnels and left to separate in a cold room at 4°C overnight. The lower phase and interface are collected and centrifuged at low speed. The pellet consists of the lower phase covered with particulate matter which collects at the interface; the supernatant is upper phase and is discarded. To ° ISOLATION AND PURIFICATION OF VIRUSES 51 extract the virus from the pellet, potassium chloride is added to precipitate the DS as the potassium salt, 0'2 ml of 3 M KCI per gramme of the original amount of DS solution being sufficient.

C. and KASSANIS, B. (1945) Ann. appl. Bioi. 32, 52-7. BAWDEN, F. c. and KASSANIS, B. (1951) Ann. appl. Bioi. 38, 402-10. BAWDEN, F. c. and PIRIE, N. w. (1937) Brit. J. expo Path. 18, 275-91. BAWDEN, F. C. and SHEFFIELD, F. M. L. (1944) Ann. appl. Bioi. 31, 33-40. , DOUGLAS, J. and MATTHEWS, R. E. F. (1974) Virology. 58, 334-44· BENNETT, C. W. (1927) Mich. Agric. Exp. Sta. Tech. Bull. No. 80. BENNETT, C. W. (1944) Phytopathology. 34, 77-91. BENNETT, C. W. (1949) Phytopathology. 39, 637-46. BENNETT, C.

KASSANIS, B. (1963) Adv. Vir. Res. 10, 219-55. , GIANINAZZI, s. and WHITE, R. F. (1974) 1. gen. Virol. 23, 11-16. , TINSLEY, T. w. and QUAK, F. (1958) Ann. appl. BioI. 46, 11-19. KASSANIS, B. and WHITE, R. F. (1974) 1. gen. Virol. 25, 323-4. KNIGHT, C. A. and STANLEY, W. M. (1941) 1. biol. Chem. 141, 39-49. , RABINA, s. and VAN PRAAGH, T. (1966) in Viruses of Plants. pp. 151-7. Eds. A. B. R. Beemster and J. Dijkstra. Amsterdam: North Holland Pub!. CO. LIVINGSTON, L. G. (1964) Amer. 1. Bot. 51, 950.

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