By Kenneth M. Smith C.B.E., D.Sc., Ph.D., F.R.S. (auth.)
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The resultant mixture is poured into large separating funnels and left to separate in a cold room at 4°C overnight. The lower phase and interface are collected and centrifuged at low speed. The pellet consists of the lower phase covered with particulate matter which collects at the interface; the supernatant is upper phase and is discarded. To ° ISOLATION AND PURIFICATION OF VIRUSES 51 extract the virus from the pellet, potassium chloride is added to precipitate the DS as the potassium salt, 0'2 ml of 3 M KCI per gramme of the original amount of DS solution being sufficient.
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