By Andrea Borini, Giovanni Coticchio

Oocyte cryopreservation involves vital capability benefits for humanIVF, delivering a much less ethically disputable substitute to embryo cryopreservation,simplifying and making more secure oocyte donation, and giving a chance forfertility upkeep to ladies liable to untimely ovarian failure as an effectof genetic components or chemo- or radiotherapies. Oocyte cryopreservation couldalso meet the expectancies of ladies wishing to maintain their fertility forsocial purposes. within the previous few years, advances in cryopreservationmethodologies have dramatically enhanced the potency of oocytecryopreservation, resulting in the start of over one thousand infants andchallenging the supremacy of embryo cryopreservation because the preferredform of fertility upkeep. this article has been conceived with the purpose of delivering a complete view of the state-of-the-art of oocyte cryopreservation. It covers primary thoughts of low temperature garage (controlled expense gradual cooling andvitrification), points of oocyte body structure suitable to the method ofcryopreservation, crucial organic and scientific facts, and ethicalimplications of oocyte cryopreservation, thereby delivering a completeoverview of growth during this technique in assisted copy.

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Additional resources for Preservation of Human Oocytes (Reproductive Medicine and Assisted Reproductive Techniques)

Example text

Each of these factors will now be discussed in sequence and the underlying science will be developed. The aim is to indicate an approach to the optimization of a cryopreservation protocol for any type of cell. CHOICE OF CRYOPROTECTANT Glycerol was the first cryoprotectant to be used in practice, and Lovelock (5) soon showed that its effectiveness could be explained by the simple fact that, by increasing the total solute concentration of the aqueous phase, it would reduce the amount of ice that formed at any given temperature during cooling.

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Cobo A, Kuwayama M, Perez S, et al. Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil Steril 2008; 89: 1657–64. Eroglu A, Toner M, Toth TL. Beneficial effect of microinjected trehalose on the cryosurvival of human oocytes. Fertil Steril 2002; 777: 152–8. Stachecki JJ, Cohen J, Willadsen SM. Cryopreservation of unfertilized mouse oocytes: the effect of replacing sodium with choline in the freezing medium. Cryobiology 1998; 37: 346–54.

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