By Willard Van Orman. QUINE
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Here we will focus on one aspect of root morphogenesis - cell expansion. The physiology of cell expansion during cell growth has been studied extensively (Taiz, 1984; Cosgrove and Kniefel, 1987). , 1993) Our goal it to use a genetic approach to dissect the processes involved in cell expansion in roots of Arabidopsis thaliana and to reveal the mechanistic and genetic basis for specific expansion patterns. , 1993). NATO ASISeries, Vol. H 81 Plant Molecular Biology Edited by G. Corum and P. Puigdommech Ii:) Springer-Verlag Berlin Heidelberg 1994 32 MATERIAL AND METHODS Plant strains.
Hypocotyl of the seedling. Such a model predicts that the complete root derives from the meristematic initials. In order to test which of these alternative developmental scenarios is happening, we performed clonal analysis. ), were used. These plants contain a T-DNA insert containing a 35S CaMV promoter, a maize Ac element, and B-glucuronidase (GUS) coding sequences. Upon excision of the Ac element in embryonic cells, clones of descendant cells can be formed in which GUS activity can be histologically detected.
1993). , 1993). Crosses were performed as described by Benfey et al, (1993). Growth measurements and cell area calculations For measurement of the root length, seedlings were photographed at roughly 24 hour intervals with a 35 rnrn camera under a Nikon stereo microscope, using Kodak Ektachrome 160T film. The root length was measured from the projected slides with a curvimeter (run-mate TM, Holland). 7%) : ethanol (50%) : acetic acid (5%» and mounted with glycerol on microscope slides. Phenotypes were analyzed with a Leitz Laborlux S compound microscope.