By Paul B. S. Clarke (auth.), Professor Paul Brian Sydenham Clarke, Professor Maryka Quik, Professor Dr. Franz Adlkofer, Professor Dr. Dr.h.c. Klaus Thurau (eds.)
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3) 10 flg of aBTX receptors purified from COL. 4) 7 flg of a-BTX receptors purified from retina. 97 67 67 57 52 43 31 - In both purified receptors the 57000 Mr peptide is recognized by the anti-a7 and anti-a8 Abs. In order to quantify the number of the a-BTX binding subunits, we performed binding experiments on the receptors bound to the a-BTX-Sepharose column. The binding of 125I_a_ BTX to immobilized receptors detects approximately only 50% of the receptors present in solution after elution with the agonist carbamylcholine, thus indicating that the a-BTX binding subunit in both receptors is present in more than one copy per receptor.
Brian Gilbert for the gift of cyclosporin A, Drs. Andres Maricq and David Julius for the gift of SHTj receptor subunit eDNA, and Dr. Mark Perin for the gift of tag1 antibody. References 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. Sargent PB. The diversity of neuronal nicotinic acetylcholine receptors. Ann. Rev. Neurosci. 1993; 16: 403-443. Patrick JW. Neuronal nicotinic acetylcholine receptor diversity. NIDA Res. Monogr. 1992; 126: 4-13. Clarke PB. The fall and rise of neuronal alpha-bungarotoxin binding proteins.
Of course, it cannot be excluded that the transient but frequently repeated blood pressure elevations, may also contribute to endothelial injury. This however seems to be doubtful in view of the often physiologically changing blood pressure episodes during the day, because of physical and emotional stress which occur quite independent of smoking. It is nicotine in cigarette smoke which plays the decisive role in the stimulation of the cardiovascular system by releasing catecholamines. , blood pressure may even rise extremely due to this mechanism (2).