By Sergei V. Konev
Fluorescence and phosphorescence are proving to be super delicate probes for elucidating conformation of proteins and nucleic acids and for learning molecular interactions. more moderen instrumentation and strategies preach nice promise for the way forward for those luminescence equipment in biopolymer learn. It needs to be famous, notwithstanding, that the invention that sure amino acids, purines, and pyrimidines emit fluorescence or phosphorescence is comparatively fresh, taking place in the final decade. Professor Konev is among the pioneers within the program of those tactics to biopolymers and is very certified to put in writing approximately this topic. This booklet, notwithstanding written principally as a monograph of the author's personal contributions, can be an exceptional assessment of the topic. Of specific curiosity are the references to many vital Russian papers during this box that have now not been well-known within the Western literature. it really is obvious from this publication that fluorescence and phosphorescence equipment are getting used approximately as largely in Russia as somewhere else on this planet and that we mustn't ever disregard those im portant contributions. Konev's experiences on protein fluorescence were widely known. it's of curiosity to benefit approximately those and different of his purposes. The final a part of the ebook, which bargains with fluorescence as a method to probe into the constitution and conforma tion of macromolecules in intact cells, is finest. other than released symposia this booklet is the 1st written particularly approximately luminescence of biopolymers. Sidney Udenfriend Bethesda. Maryland may perhaps, 1967 v CONTENTS creation . • • . . . . . . . . . • . . . . . . . . . .
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Additional resources for Fluorescence and Phosphorescence of Proteins and Nucleic Acids
This part of the fluorescence of the film may be 100% polarized. and its intensity. directly proportional to the number of quanta absorbed by the 1Lb oscillator in relation to the total absorption by the two oscillators. In other words. the polarization spectrum obtained in this case reflects the relationship P"-=f( elLbA ) elLa A elLb A + 33 ELECTRONIC EXCITED STATES OF MONOMERS and is actually the relative absorption spectrum of the negative oscillator. We shall now consider the results obtained from experiments.
40·noJcm- 1 ~ Fig. 13. Spectra of DL-tryptophan in polyvinyl alcohol film at 20°C: (1) absorption spectrum, (2) fluorescence spectrum, (3) absorption polarization spectrum of fluorescence (recorded at 330 mil). (4) the same (recorded at 295 mil), (5) emission polarization spectrum of fluorescence (excitation 265 mIL and t =-196°C). (6) phosphorescence spectrum, (7) absorption polarization spectrum of phosphorescence (recorded at 460 mil), (8) emission polarization spectrum of phosphorescence (excitation 265 mil), (9) spontaneous emission polarization spectrum of phosphorescence (excitation 265 mil).
Tryptophan in the crystalline state differs considerably from tryptophan in solution not only in the low intensity of the phosphorescence, but also in the fact that the phosphorescence spectrum is shifted in the long-wave direction to 500 mJ-L and loses its structure. Concentrated aqueous solutions of tryptophan give a similar picture. The addition of substances which bind the hydrogen of the imino group and thus prevent association (formaldehyde) leads to restoration of the ordinary phosphorescence of monomeric tryptophan.