By A. Roy (Eds.)
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50. Shmookler R e i s , R . J . , Lumpkin, C . K . , R i a b o w o l , K . T . a n d G o l d s t e i n , S . , N a t u r e 3 0 1 , 394 (1983). S h m o o k l e r R e i s , R . J . and G o l d s t e i n , S . , J . Biol. Chem. 2 5 8 , 9078 (1983). L e d e r , P . , S c i e n t i f i c A m e r i c a n 2 4 6 , 102 ( 1 9 8 2 ) . D e L a p , R . J . and R u s h , M . G . , P r o c . N a t l . A c a d . S e i . USA 7 5 , 5 8 5 5 (1978). B e r t e l s e n , A . H . , Humayun, M . Z . , L i p p m a n , A .
Exp. C e l l R e s . 4 2 , and Goldstein, and G o l d s t e i n , 3949 (1982). P o n t e n , J . and 673 (1966). Macieira-Coelho, Α . , Mech. , Cell Proc. 21, Natl. , D e v . C>, 341 (1977). Y a n i s h e v s k y , R . , M e n d e l s o h n , M . L . , M a y a l l , B . H . and C r i s t o f a l o , V . , J. C e l l P h y s i o l . 8 4 , 165 ( 1 9 7 4 ) . H a r l e y , C . B . , S h m o o k l e r R e i s , R . J . and G o l d s t e i n , S . J. T h e o r e t . B i o l . 9 4 , 1 ( 1 9 8 2 ) . M a n u e l i d i s , L .
One m u s t c h a r a c t e r i z e them b y r e s t r i c t i o n enzyme a n a l y s i s and c l o n e t h e monomeric subunit in i t s e n t i r e t y . T h i s i s not always easy s i n c e o f t e n j u s t a f e w m i c r o g r a m s a r e a v a i l a b l e from s e n e s c e n t m y c e l i a and i t i s d i f f i c u l t t o d e t e r m i n e unique r e s t r i c t i o n s i t e s s u i t a b l e f o r c l o n i n g . This h a s b e e n d o n e f o r f o u r s e n e s c e n t m i t o c h o n d i r a l DNAs and t h e s e a r e shown i n F i g u r e 4 .