By F. Korte

Nucleic Acids, Proteins and Carbohydrates

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105 m A. , Vol. 12, Part B, p. 212, Academic Press, New York, London 1968. 1:1 Introduction Since the discovery of this enzyme, it has been a most useful tool for the synthesis of various homopolymers and copolymers with the high molecular weight of 30,000 to 2,000,000loe. More­ over, it could be applied to the synthesis of oligomers with defined sequences by the im­ provement of the polymerizing conditions107 and the development of techniques for the sepa­ ration and purification of the desired oligomer from the synthesized products, which usually are a mixture of oligomers with similar sequence.

234, 240 (1971). 94 /. P. Leis, I. Berkower, J. Hurwitz, Proc. Nat. Acad. Sci. S. 70, 466 (1973); D. P. Grandgenett, M. Green, J. Biol. Chem. 249, 5148 (1974). 95 D. Baltimore, D. F. Smoler, J. Biol. Chem. 247, 7282 (1972). 98 ΛΓ. , Vol. 4, p. 313, Academic Press, New York, London (1971). 97 A. Bernardi, G. , Vol. 4, p. 329, Academic Press, New York, London (1971). Both enzymes have been widely used for nucleic acid research because of the sequential degrada­ tion with distinct polarity of 3'—>5' or 5'—>3'.

Bertsch, J. Biol. 14 T. Kornberg, A. Lockwood, A. Worcel, Proc. Nat. Chem. 224» 2996 (1969). Acad. Sci. S. 71, 3189 (1974). 1:8 daltons which code for only eight to ten gene products. g. coat proteins, so that these viruses have to use the replication machinery of the host cell for their own progeny production. The study of DNA replication of these small coliphages combined with the biochemical and genetic analysis of the E. coli host enzymes and host factors which are involved in DNA synthesis has greatly facilitated our understanding of DNA replication in vitro and in vivo.

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